Category
Laboratory Immunology
Typical Use
Analyze and sort cells by fluorescence and light scattering properties.
Description
An instrument that passes cells in single file through laser beams to measure size, granularity, and fluorescent markers, enabling immunophenotyping, cell cycle analysis, and sorting for research and clinical applications such as leukemia classification and immune monitoring. Flow cytometry provides multiparametric data on heterogeneous cell populations and supports diagnostic, prognostic, and therapeutic decision making when combined with validated antibody panels and standardized gating strategies. Operation requires trained technologists, calibration beads, and rigorous compensation for spectral overlap to ensure accurate results, and sorted cells may be used for downstream functional assays or molecular testing. Flow cytometry is a powerful tool in hematology, immunology, and translational research with broad clinical impact.